《植物生理学报》 2017, 53(3): 331-338

植物中转录抑制机制的研究进展

孙佩文¹, 吕菲菲², 徐艳红^1,*, 魏建和^1,2,*

¹中国医学科学院北京协和医学院药用植物研究所, 北京100193; ²中国医学科学院北京协和医学院药用植物研究所海南分所(海南省南药资源保护与开发重点实验室), 海南万宁571533

通信作者：徐艳红；E-mail: wjianh@263.net; xuyanhong99@163.com

摘　要：

基因表达的激活和抑制是构成生机盎然、绚烂多彩的植物世界的核心机制。转录调控是基因表达至关重要的环节, 其中转录抑制是通过阻碍目的基因的活化来实现基因转录负调控的。目前的研究已揭示出了多种植物基因转录激活的分子调控机制, 但转录抑制方面的内容还相对较少。本文将从转录前起始复合物、染色质修饰重构、蛋白磷酸化、转录抑制子等几方面的调控作用着眼, 概述在植物生长发育及胁迫防御过程中植物基因转录抑制的研究进展, 重点阐述转录抑制子的抑制与解除作用, 为进一步解读此调控“密码”提供参考依据, 并为我们深入了解基因表达调控, 特别是植物防御反应的分子机制提供参考。

关键词：转录调控; 抑制机制; 转录抑制子

收稿：2016-08-26   修定：2017-01-05

资助：国家自然科学基金(81173539和81573525)和海南省重大科技专项(ZDKJ2016004)。

Advances in transcriptional repression mechanisms in plant

SUN Pei-Wen¹, LÜ Fei-Fei², XU Yan-Hong^1,*, WEI Jian-He^1,2,*

¹Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China; ²Hainan Branch of the Institute of Medicinal Plant Development (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine), Chinese Academy of Medical Sciences & Peking Union Medical College, Wanning, Hainan 571533, China

Corresponding author: XU Yan-Hong; E-mail: wjianh@263.net; xuyanhong99@163.com

Abstract:

Activation and inhibition of gene expression are the core mechanisms for forming the lifeful and splendid plant world. The regulation of gene expression at transcriptional level is a crucial process, in which the transcriptional repression is achieved by inhibiting the target gene activation. Although the gene activation mechanisms involved in plant defensive response and development have been revealed a lot, little is known about the gene repression mechanism. So we focus on the research in the field of transcriptional repression in plant development and defensive response from the perspectives of pre-initiation complex, chromatin modification and remodeling, protein phosphorylation, and transcriptional repressors. Particularly, the gene inhibition caused by the transcriptional repressors and its release mechanisms are described in detail. This review could provide useful information for further interpreting the code of plant transcriptional regulation, and improve our understanding of the gene expression and regulation, as well as the molecular mechanisms governing plant stress response.

Key words: transcriptional regulation; inhibition mechanism; transcriptional repressor